I began my internship at the Salk Institute for Biological Studies in Dr. Greg Lemke’s lab on May 27th. I am working under the supervision of a postdoctoral fellow named Lawrence Fourgeaud. I spent the first couple of days getting oriented and settling in. In my training, we reviewed how to work safely and conscientiously in a lab. I then learned how to handle and work with mice ethically and effectively through hands-on instruction and an online training course. As I finished up my training at the end of the first week, I began doing lab work. One of the first experiments I did was run a qPCR. qPCR is a method to look at the level of expression of specific genes in cells and tissues.
Another technology I learned about is in vivo two-photon microscopy. We are working in collaboration with a postdoctoral researcher in another lab here at Salk (Nimmerjahn lab). We want to image microglia cells in vivo (ie. in an anesthetized animal) using this technique.